•  
  •  
 

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is an opportunistic organism that affects humans with a number of critical infections by developing biofilm. MRSA cells embedded in biofilm frequently exhibit drug tolerance and emerge as a serious threat to public healthcare. Towards the effective control of the biofilm-associated threats of MRSA, cuminaldehyde and tobramycin were used independently as well as in combination. Cuminaldehyde and tobramycin individually as well as in combination showed effective antimicrobial activity against the clinical isolates of MRSA. The fractional inhibitory concentration (FIC) index of cuminaldehyde (an essential oil) and tobramycin (an aminoglycoside antibiotic) against clinical isolates of MRSA was found to be ˂ 0.5, indicating a strong synergy between them. The antibiofilm potential of the mentioned compounds was tested at the sub-MIC doses wherein cuminaldehyde and tobramycin together could show significant antibiofilm potential against the clinical isolates of MRSA without affecting microbial growth. Further analysis revealed that the combination of cuminaldehyde and tobramycin could accumulate reactive oxygen species (ROS) that resulted in the inhibition of biofilm formation as well as disintegration of pre-existing biofilm. Thus, the findings revealed that combinatorial applications of cuminaldehyde and tobramycin could be used for the effective control of MRSA-linked biofilm challenges even on catheter surface. Hence, the combinatorial application of cuminaldehyde and tobramycin could be used for the effective management of microbial infections involving MRSA.

Download

Share

COinS